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This research was focused to develop and evaluate the effectiveness of a multimedia learning environment with the purpose of increasing learners’ knowledge, awareness as well as perceived motivation towards learning of autism behavioral symptoms. An Interactive Multimedia Learning Environments (IMLE) was developed by integrating relevant learning theories and multimedia principles to provide learners with an interactive content experience. The objective of this research was to study the effects of IMLE as measured by Knowledge Awareness Scale (KAS) in terms of Design Strategies, Gender, Learning Styles and perceived motivation. This research employed a quasi-experimental research with purpose to investigate the effects of two presentation modes: Presentation Mode 1 (Modality Principle) and Presentation Mode 2 (Redundancy Principle). The sample of this study consists of special education pre service teacher who were taking special education courses at local public universities. The total population size of 550 was selected and based on Krejcie and Morgan (1970) table; around 225 samples was selected for the final treatment. The final data were compiled and analyzed using Statistical Packages for the Social Sciences (SPSS) version 19. Analysis of Covariance (ANCOVA), Multivariate Analysis of Covariance (MANCOVA) and Pairwise Comparison were used for inferential statistical analysis. In general, the findings from this study shows a significant increased in the amount of knowledge and awareness of respondents towards the learning material. This learning environment can help to shorten the process in identifying autism symptoms in the future as the technology of computers evolved every day. The learning theories and multimedia principles used in this study were successfully managed to achieve the research objectives and research hypothesis. Furthermore the gain scores showed by the presentation modes was a result of the effectiveness of Knowledge Awareness Scale (KAS) instrument used during the treatment. Nevertheless, further improvements and amendments are necessary to make this study more effective. The use of more reliable and current learning theories and multimedia principle are needed as to strengthen the development of learning material.