The aim of this research is to determine the prospective primary teachers’ knowledge levels about basic science concepts. For this purpose, a survey research methodology was used in this research. The sample of the research consists of 35 prospective primary teachers who are educated in the 3^rd grade of Muş Alparslan University Education Faculty. In order to collect data in the research, Science Test (ST), which consists of 15 open-ended questionnaires containing the concepts of physics, chemistry and biology lessons which are the subfields of science, was used. For the validity of the test, 3 experts were consulted. The consistency of the scorer was examined for its reliability. It was used percentage and frequency values of descriptive statistics for analyzing the data. Findings from the research show that some prospective primary teachers have various conceptual misconceptions about the physics, chemistry and biology concepts and also do not know some concepts. It is thought that in these results emerging in the research, science concepts are generally abstract and that prospective primary teachers’ academic achievement level are too low.