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The total of 20 isolates of Acetia Acid Bacteria were isolated from 21 specimens of Flower that collected in Suan Sunandha Rajabhat University, Bangkok, Thailand by enrichment culture method. The 5 enrichment culture media, Glucose-ethanol-acetic acid medium, Sorbitol medium, Dulcitol medium, Mannitol medium and Methanol medium at pH 3.5 were used to promote growth of Acetic Acid Bacteria and the Glucose-Ethanol-Calcium-Carbonate agar was use to screen acid producing bacteria. The morphology and biochemical test, catalase test, oxidation-fermentation test, oxidation of acetate-lactate test and culturing on the different carbon sourse were used to identify all of Acetic Acid Bacteria in this study. All of the 20 isolates were gram negative, rod shape, aerobic acid producing bacteria, oxidase negative, catalase positive. All of them were identify as Genus Asaia because of their oxidation of acetate and lactate were weak and their growth on glutamate agar and 30 % Glucose medium were positive.