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This study focuses on the development and validation of a teacher made analytic and holistic rubric guide in assessing Concept Cartoons in teaching science lessons. This study is a descriptive-developmental type of research. In validating and testing the reliability of the developed rubric guides, science teachers and experts, and students from Quezon City Polytechnic University served as respondents. Three analytic and three holistic rubric guides to assess three different forms of Concept Cartoons were developed, used and subjected for validity and reliability test. Results showed that the developed analytic and holistic rubric guides were valid and reliable. In conclusion, valid and reliable analytic and holistic rubric guides are important in giving a conclusive analysis of student’s learning. With the use of valid and reliable rating scales strengthen the claims that concept cartoons help teachers in identifying and correcting students’ misconceptions.