Curiosity about the accessibility of microbiology literacy has increased in recent times. This study aims to comprehensively identify trends in microbiology virtual through virtual media. Virtual simulations and laboratories provide access to safe and economical laboratory work experiences, without the constraints of space and time. The visualization used in this study is supported by Biblioshiny from RStudio, utilizing the Scopus database as a reference source. We conducted a document search on the implementation of virtual media in the field of microbiology. Overall, the comprehensive analysis shows that publications in this field have experienced annual growth, with an average increase of 10.47%. scientific production on this topic saw a significant rise in early 2017, with the highest peak occurring in 2021. The Journal of Microbiology and Biology Education ranks as the journal with the highest contribution, and the University of Tokyo as the leading affiliation in terms of publication productivity. The analysis results in this study provide a comprehensive report and evolving research trends. This information helps researchers and educators identify potential areas for future research. Virtual media in microbiology learning opens opportunities to reach students from various economic backgrounds, allowing for more inclusive and equitable learning experiences. Based on these findings, it is recommended that educational institutions and technology developers collaborate in the further development of advanced laboratory simulations and their integration into curricula. Furthermore, additional research should be conducted to evaluate the effectiveness of virtual media on learning outcomes. It is also crucial to provide training for educators and to ensure equitable access to technological infrastructure, thereby guaranteeing equal opportunities for all students.